| Team name | Count of racers | Category | |
| 2M | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| Bajkeři Jičína | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| Bezteamu | 2/2 | PAIRS MIX (only course A - 100 km) |
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| Bike Team Žebrák | 2/2 | PAIRS Men (only course A - 100 km) |
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| Cycology | 2/2 | PAIRS Men (only course A - 100 km) |
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| Decathlon Czech cycling team | 2/2 | PAIRS MIX (only course A - 100 km) |
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| Dr.Uhli | 2/2 | PAIRS MIX (only course A - 100 km) |
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| Duo Continental riders | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| EMERGE / KOLOFIX | 2/2 | PAIRS Men (only course A - 100 km) |
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| EMERGE / KOLOFIX | 2/2 | PAIRS Men (only course A - 100 km) |
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| Fahrwerk Werksteam | 2/2 | PAIRS Men (only course A - 100 km) |
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| Fly4Sport Racing | 2/2 | PAIRS Men (only course A - 100 km) |
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| Haven and BEKO BIKE | 2/2 | PAIRS Men (only course A - 100 km) |
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| Merida Bikeranch Team | 1/2 | PAIRS Men (only course A - 100 km) |
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| Merida Bikeranch Team | 2/2 | PAIRS Men (only course A - 100 km) |
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| MTB HELLFISH MOST | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| MTB Věncovka Racing Team | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| Povltavští bajkeři | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| Rohozec Amix Team | 2/2 | PAIRS Men (only course A - 100 km) |
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| Ski a Bike centrum Radotín | 2/2 | PAIRS Men (only course A - 100 km) |
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| Sport Invest MTB team | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| Sport Invest Team | 2/2 | PAIRS Men (only course A - 100 km) |
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| STS EXTREME STRZELIN GROM-BIKE TEAM | 2/2 | PAIRS Men (only course A - 100 km) |
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| Team Pirna | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| UNLIMITED KM TRADING CZ | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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| VELOKLASIK - Café du Cycliste | 2/2 | PAIRS Men MASTERS 40+ (only course A - 100 km) |
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